| | Horse | Trainer | Age | Weight | Jockey | Odds | Bookie |
| An Accordion Improving young chaser who won two big races at the end of 2008 season including at the Cheltenham Festival. If improvement continues can be a strong player in 2009 National. | David Pipe | 7 | 0-0 | | 33/1 |  |
 | Bothar Na Has put in some decent performances over the National fences but might need to go up the weights somewhat to be sure of making it into the handicap. | Willie Mullins | 9 | 0-0 | | 40/1 |  |
 | Butlers Cabin Looked to be going well when fell at Bechers in 2008. Stable due a change of luck in this race. | Jonjo ONeill | 8 | 0-0 | | 20/1 |  |
| Character Building Was well fancied for 2008 National but an injury kept him out of the race. Likely to be prepared for the 2009 race. | John Quinn | 8 | 0-0 | | 37/1 |  |
 | Chelsea Harbour Won a National trial at Punchestown in 2008 and completed in his own time in the great race itself. Likely to try again in 2009. | Thomas Mullins | 8 | 0-0 | | 50/1 |  |
 | Cloudy Lane Disappointed in 2008 National and hard to imagine he will fare any better with more weight in 2009. | Donald McCain Jnr | 8 | 0-0 | | 33/1 |  |
 | Comply Or Die Jumped brilliantly to land the National in 2008. Can he become the first horse to retain his crown since Red Rum? | David Pipe | 9 | 0-0 | | 20/1 |  |
| Denman 2008 Gold Cup Winner has been mentioned as a National possible. What a prospect! | Paul Nicholls | 8 | 0-0 | | 16/1 |  |
| Garde Champetre Cross Country specialist who could be diverted to the Grand National. Would be very interesting if given the go ahead. | Enda Bolger | 9 | 0-0 | | 33/1 |  |
| Gungadu 2008 Racing Post Chase winner has same connections as Denman and may be given his chance if that horse does not take part. | Paul Nicholls | 8 | 0-0 | | 33/1 |  |
| Gwanako Put up a terrific performance to win the 2008 Topham as a five year old. Bags of potential but a big ask to win the National at six years old. | Paul Nicholls | 5 | 0-0 | | 25/1 |  |
| Halcon Genelardais Didn't manage to win in 2007/8 but put up some top class weight carrying performances. One of the best staying chasers in training. | Alan King | 8 | 0-0 | | 33/1 |  |
| Hear The Echo 2008 Irish Grand National winner will be looking to uphold the good record of winners of that race at Aintree. | Mouse Morris | 7 | 0-0 | | 25/1 |  |
| Himalayan Trail Improving young stayer. Won the Midland Grand National in 2008 so has the required stamina for Aintree. | Sue Smith | 9 | 0-0 | | 25/1 |  |
| Iris De Balme Shock winner of the 2008 Scottish National will be looking to confirm that his victory at Ayr was no fluke by running well at Aintree. | Sean Curran | 8 | 0-0 | | 25/1 |  |
 | Irish Raptor Runner-up to Gwanako in the Topham and will be suited to the extra distance of the National if making the weights. | Nigel Twiston-Davies | 9 | 0-0 | | 40/1 |  |
 | King Johns Castle Gallant runner-up in 2008. Will be aimed at the race again in 2009. | Arthur Moore | 9 | 0-0 | | 25/1 |  |
 | Lami On a very long losing run and has disappointed twice in the National already. | Francois Doumen | 9 | 0-0 | | 50/1 |  |
| Miko De Beauchene Gutsy winner of the Welsh National in 2008. Could be the type of fairystory winner that the Grand National is famous for. | Robert and Sally Alner | 8 | 0-0 | | 25/1 |  |
 | Mon Mome Decent stayer who didn't really look at his best in 07/08 and may improve again with the benefit of antother years experience. | Venetia Williams | 8 | 0-0 | | 50/1 |  |
 | Mr Pointment Brilliant display over the National fences in 2007 Becher but question marks about his fitness. | Paul Nicholls | 9 | 0-0 | | 50/1 |  |
| Old Benny Won the National Hunt Chase at the Cheltenham Festival but looks as if he could do with a few more miles on the clock before tackling Aintree. | Alan King | 7 | 0-0 | | 25/1 |  |
 | Patsy Hall Disappointing in 07/08 but dangerous to underestimate a Tony Martin horse. Could easily come back to form. | Tony Martin | 8 | 0-0 | | 40/1 |  |
 | Royal County Star Runner up in Irish Grand National. Comes from powerful stable of Tony Martin | Tony Martin | 9 | 0-0 | | 33/1 |  |
 | Simon Failed to get round for the second year in succession and it may just be that his best chance has gone. | John Spearing | 9 | 0-0 | | 33/1 |  |
 | Slim Pickings Third in 2007 and fourth in 2008. Sadly it looks like his stamina just doesn't see him home. | Tom Taaffe | 9 | 0-0 | | 33/1 |  |
 | Snowy Morning Cracking effort to finish third in 2008. Will be trained specifically for this race in 2009. | Willie Mullins | 8 | 0-0 | | 20/1 |  |
 | Turko Fell in 2008 National when well fancied by his powerful stable. Likely to return in 2009. | Paul Nicholls | 5 | 0-0 | | 33/1 |  |